Nucleic Acids

The Burton Assay for DNA.- DABA Fluorescence Assay for Submicrogram Amounts of DNA.- Preparation of RNase-Free DNase by Alkylation.- The Isolation of Satellite DNA by Density Gradient Centrifugation.- The Isolation of High Molecular Weight Eukaryotic DNA.- Preparation of Lyophilized Cells to Preserve Enzyme Activities and High Molecular Weight Nucleic Acids.- Agarose Gel Electrophoresis of DNA.- Autoradiography of Gels Containing 32P.- Detection of Specific DNA SequencesThe Southern Transfer.- The Extraction and Isolation of DNA from Gels.- One-Dimensional Electrophoresis of Nucleic Acids in Agarose Using Denaturation with Formaldehyde and Identification of 3H-Labeled RNA by Fluorography.- Gel Electrophoresis of RNA in Agarose and Polyacrylamide Under Nondenaturing Conditions.- The Extraction of Total RNA by the Detergent and Phenol Method.- RNA Extraction by the Proteinase K Method.- RNA Extraction by the Guanidine Thiocyanate Procedure.- The Purification of Poly(A)-Containing RNA by Affinity Chromatography.- Messenger RNA Fractionation on Neutral Sucrose Gradients.- The Estimation of mRNA Content by Poly(U) Hybridization.- DNA Directed in Vitro Protein Synthesis with Escherichia coli S-30 Extracts.- In Vitro Translation of Messenger RNA in a Wheat Germ Extract Cell-Free System.- In Vitro Translation of Messenger RNA in a Rabbit Reticulocyte Lysate Cell-Free System.- Immunoprecipitation of in Vitro Translation Products with Protein A Bound to Sepharose.- In Vitro Continuation of RNA Synthesis Initiated in Vivo.- Synthesis of Double-Stranded Complementary DNA from Poly(A)+mRNA.- Plasmid DNA Isolation by the Cleared Lysate Method.- Plasmid DNA Isolation (Sheared Lysate Method).- Small-Scale Plasmid DNA Preparation.- Preparation of Chromosomal DNA from E. coli.-Preparation and Assay of Phage Lambda.- Preparation of Phage Lambda DNA.- The Use of Restriction Endonucleases.- Ligation of DNA with T4 DNA Ligase.- The Use of Alkaline Phosphatase to Prevent Vector Regeneration.- Bacterial Transformation.- Bacterial Transformation (Kushner Method).- In Vitro Packaging of DNA.- Yeast Transformation.- Radiolabeling of DNA by Nick Translation.- Radiolabeling of DNA Using Polynucleotide Kinase.- Radiolabeling of DNA with 3? Terminal Transferase.- Radiolabeling of DNA with the Klenow Fragment of DNA Polymerase.- Identification of Recombinant Plasmids by In Situ Colony Hybridization.- Identification of Recombinant Phages by Plaque Hybridization.- Plasmid Screening Using Single Colony Lysates.- Immunological Detection of Gene Expression in Recombinant Clones.- The Isolation of Minicells.- The Isolation of Maxicells.- The Identification of Gene Products in Minicells and Maxicells.- Molecular Cloning in Bacteriophage Lambda and in Cosmids.- DNA Transformation of Mammalian Cells.- Chemical Cleavage (Maxam and Gilbert) Method for DNA Sequence Determination.- Gel Electrophoretic Analysis of DNA Sequencing Products.- DNA Sequence Determination Using Dideoxy Analogs.